Custom Taqman Assay Design Tool Cadt On The Life Technologies
Custom Applied Biosystems TaqMan Expression assays are the custom version of our predesigned real-time PCR 5' nuclease TaqMan assays for relative quantification of RNA expression. Simply provide us with your target sequence of interest, or submit primer/probe sequences of your own design, and soon you'll be ready to perform RNA expression studies on any coding or non-coding RNA in any organism.
- Review our Design and Ordering Guide (PDF) for best results.
- Use the Custom TaqMan Assay Design Tool to input sequences, submit for design, and order custom assays.
Each custom assay is a mix of forward primer, reverse primer, and FAM or VIC dye-labeled TaqMan MGB probe. Designed to run under universal oligo concentration and thermal cycling conditions for two-step RT-PCR, these assays are simple to use. Just add TaqMan Master Mix and your cDNA sample to generate sensitive, reproducible, and truly quantitative RNA expression data.
Order custom assays
Looking to order a custom TaqMan Gene Expression Assay with a dye other than FAM or VIC?
Visit our Specialty TaqMan Assays & Oligos webpage to learn more.
How to Order
This product can be purchased by visiting AppliedBiosystems.com. Direct ordering through the Life Technologies website will be available soon.
Comparison of Custom and Custom Plus TaqMan Gene Expression Assay products
There are two custom assay options to meet your RNA research needs: Custom TaqMan Gene Expression Assays and Custom Plus Applied Biosystems TaqMan RNA Assays. Both allow you to submit a target sequence to our TaqMan Assay design pipeline, which uses your input sequence to design custom primer and probe sequences. See the key differences between the two options below:
Custom Plus TaqMan RNA Assays are designed using the full bioinformatic power of the TaqMan Assay design pipeline, which uses proprietary algorithms that have generated millions of assay designs and is regarded as the benchmark in the industry for qPCR design. If you do not need bioinformatics or QC checks performed on your sequences, then our standard Custom TaqMan Gene Expression Assays will meet your needs.
Target sequence submission is easy using the Custom Assay Design Tool (CADT). Simply select your bioinformatic analysis and assay specificity preferences, and enter your target sequence. Sequence submissions are completely confidential; we will not share your target sequences or assay sequences with any third parties.
If you do not have a pre-defined input sequence, the CADT will help you to search for sequences by keyword (gene symbol, accession number, etc.) or by genomic location in a broad range of the most popular and well-studied model species ranging from human and mouse to pig, dog, cow, Arabidopsis, and more.
Order custom assays
| Sku | Product Name | Size |
|---|---|---|
| 4331348 | Custom TaqMan® Gene Expression Assay, SM FAM | 360 reactions, 20x |
| 4332078 | Custom TaqMan® Gene Expression Assay, MED FAM | 750 reactions, 20x |
| 4332079 | Custom TaqMan® Gene Expression Assay, LG FAM | 2900 reactions, 60x |
| 4441114 | Custom Plus TaqMan® RNA Assay, SM FAM | 360 reactions, 20x |
| 4441117 | Custom Plus TaqMan® RNA Assay, MED FAM | 750 reactions, 20x |
| 4441118 | Custom Plus TaqMan® RNA Assay, LG FAM | 2900 reactions, 60x |
| 4448487 | Custom TaqMan® Gene Expression Assay, SM VIC PL | 360 reactions, 20x |
| 4448508 | Custom TaqMan® Gene Expression Assay, SM VIC | 360 reactions, 20x |
| 4448488 | Custom TaqMan® Gene Expression Assay, MED VIC PL | 750 reactions, 20x |
| 4448509 | CustomTaqMan® Gene Expression Assay, MED VIC | 750 reactions, 20x |
| 4448492 | Custom TaqMan® Gene Expression Assay, LG VIC PL | 2900 reactions, 60x |
| 4448510 | Custom TaqMan® Gene Expression Assay, LG VIC | 2900 reactions, 60x |
| 4448511 | Custom Plus TaqMan RNA Assay SM VIC PL | 360 reactions, 20x |
| 4448514 | Custom Plus TaqMan RNA Assay SM VIC | 360 reactions, 20x |
| 4448512 | Custom Plus TaqMan RNA Assay MED VIC PL | 750 reactions, 20x |
| 4448515 | Custom Plus TaqMan RNA Assay MED VIC | 750 reactions, 20x |
| 4448513 | Custom Plus TaqMan RNA Assay LG VIC PL | 2900 reactions, 60x |
| 4448516 | Custom Plus TaqMan RNA Assay LG VIC | 2900 reactions, 60x |
Approximate delivery times are 4-6 days in the US and 6-10 days in Europe.
- Bergmann O et al. (2011) Identification of cardiomyocyte nuclei and assessment of ploidy for the analysis of cell turnover. Exp Cell Res 2, 188-194.
- Hevir N, Sinkovec J, Rizner TL (2011) Disturbed expression of phase I and phase II estrogen-metabolizing enzymes in endometrial cancer: lower levels of CYP1B1 and increased expression of S-COMT. Mol Cell Endocrinol 1, 158-167.
- Colazzo F et al. (2011) Extracellular matrix production by adipose-derived stem cells: implications for heart valve tissue engineering. Biomaterials 1, 119-127.
Custom Taqman Assay Design Tool Cadt On The Life Technologies
Source: https://www.thermofisher.com/id/en/home/life-science/pcr/real-time-pcr/real-time-pcr-assays/taqman-gene-expression/custom-taqman-gene-expression-assays.html
Posted by: perkinssweves.blogspot.com
0 Response to "Custom Taqman Assay Design Tool Cadt On The Life Technologies"
Post a Comment